Fans theorize the possibility of Gwen Stacy from Spider-Man: Across the Spider-Verse being Transgender due to potential sub-text.
Johnny on X: Why are people surprised when this was blatantly set up!? lol / X
Spider-Man: Across the Spider-Verse fans see Gwen Stacy as an LGBTQ hero - Vox
Why did Gwen have to lie about her name in Spider-Man: Into the Spiderverse? - Quora
Brendan Hodges on X: Those policing if Gwen is literally trans are missing the point. As shown in THE MATRIX, queer and trans readings are not incumbent on canon or even intent.
Gwen is blatantly a trans woman in this movie: Across the Spider-Verse Spider-Gwen Fan Theory Changes Everything
Gwen Stacy (Spider-Verse) - Wikipedia
Spider-Man: Across the Spider-Verse' may have a trans character. Here's why fans say it matters
Is Gwen Stacy trans in Across the Spider-Verse? All the evidence
Gwen Stacy in 'Spider-Man: Across the Spider-Verse' might be the trans ally superhero you were waiting for - India Today