Downregulation of microRNA-34 induces cell proliferation and

Malignant mesothelioma (MM) is an aggressive tumor with a dismal prognosis, and the molecular alterations involved in this disease remain unknown. We previously reported that microRNA-34s (miR-34s) are methylated and downregulated in MM and may play an important role in the carcinogenesis of MM. In this study, we downregulated miR-34s in human mesothelial cells to investigate the cellular effect of miR-34 knockdown. For the cell study, we used LP-9, a human mesothelial cell line, and three human primary-cultured mesothelial cell lines. RNA-based miR-34a, -34b and -34c inhibitors were transfected into these cells, and their effects on proliferation and invasion were evaluated. A scramble RNA oligonucleotide was used as a control. The protein expression status was estimated using western blotting. After miR-34 inhibitor transfection, miR-34a, -34b and -34c were downregulated in all the examined mesothelial cell lines. miR-34 inhibitor transfection significantly increased cell proliferation in all of the mesothelial cell lines, compared with the scramble control. The invasive ability also increased in the miR-34 inhibitor transfectants, compared with the scramble control, in the LP-9 cell line. Western blotting confirmed the upregulation of c-MET, phospho-c-MET, and bcl-2 proteins in LP-9 cells after miR-34 inhibitor transfection. In conclusion, our study showed that the downregulation of miR-34s induced an oncogenic phenotype in non-malignant mesothelial cells. The present study, together with the results of our previous report, strongly suggest that miR-34s play an important role in the early carcinogenic process involved in the transformation of human mesothelial cells to MM.

Interactions of the miRNAs downregulated in the colorectal cancer with

PDF) Abstract 187: Down-regulation of microRNA34 induces cell proliferation and invasion of human mesothelial cells

MicroRNA-34a Promotes Renal Fibrosis by Downregulation of Klotho in Tubular Epithelial Cells - ScienceDirect

TP53 induces miR-15/16 and miR-34 expression. On the contrary

microRNA-mediated cell-to-cell communication via extracellular

Genes & Cancer Oncogenes in high grade serous adenocarcinoma of the ovary

Oncogenic and tumor suppressive roles of microRNAs in apoptosis and autophagy

IJMS, Free Full-Text

Frontiers Frontiers of MicroRNA Signature in Non-small Cell Lung Cancer

A comparison between the effects of over-expression of miRNA-16 and miRNA-34a on cell cycle progression of mesothelioma cell lines and on their cisplatin sensitivity - ScienceDirect

MicroRNA-34 family: a potential tumor suppressor and therapeutic candidate in cancer, Journal of Experimental & Clinical Cancer Research

miR-34a directly targets tRNAiMet precursors and affects cellular proliferation, cell cycle, and apoptosis